Not with my template, only with templates such as silverfish and redpassion.

Lightbox works with my template for one image just fine, but when it comes to reading additional images, it doesn't look for them. The ligthbox with the other two templates sniff the additional images out with no problem. This makes me confident there should be no code manipulation in the lightbox script needed, however I might have to change code in my template script, but I don't know where or what code.